Journal: Journal of Traditional and Complementary Medicine

Article Title: Polysaccharides derived from alkali-extracted vinegar-baked Radix Bupleuri suppress hyperimmune T lymphocytes and ameliorate skin graft rejection

doi: 10.1016/j.jtcme.2024.11.006

Figure Lengend Snippet: The effect of KVBCP3 and KVBCP4 on immune cytokines and Treg cells of mice with skin allograft rejection. (A) The secretion of IL-2 in serum was determined by Elisa assay (n = 6). (B) The secretion of IL-4 in serum was determined by Elisa assay (n = 6). (C) Representative flow cytometry plots of CD4 + CD25 + Foxp3 + Treg cells after treatment with KVBCPs at the 14th day; (D) The change of the proportions of CD4 + CD25 + Foxp3 + Treg cells after treatment with KVBCPs at the 14th day (n = 3). (E) Representative flow cytometry plots of CD4 + IL-17A + cells after treatment with KVBCPs at the 14th day; (F) The change of the proportions of CD4 + IL-17A + cells after treatment with KVBCPs at the 14th day (n = 3). ∗ P < 0.05, ∗∗ P < 0.01 vs Model group, one way ANOVA.

Article Snippet: Cell stimulation and Protein Transport Inhibitor Kit (Lot: AK22752), FITC Anti-Mouse CD4 Antibody (Lot: AF21880), PE Anti-Mouse IL-17A Antibody (Lot: AF17079) were purchased from Elabscience (Wuhan, China).

Techniques: Enzyme-linked Immunosorbent Assay, Flow Cytometry

Journal: Nature communications

Article Title: Targeting IL-17A enhances imatinib efficacy in Philadelphia chromosome-positive B-cell acute lymphoblastic leukemia.

doi: 10.1038/s41467-023-44270-3

Figure Lengend Snippet: Fig. 5 | IL-17A stimulates the secretion of CXCL16 from leukemia cells, pro- moting the differentiation and migration of Th17 cells. a B220+ cells and Th17 cells in the BM niche in WT mice and BCR-ABLtTA mice (n = 3 mice per group) were subjected to immunofluorescence staining. Representative images were shown. b The numbers of Th17 cells migrating to culture medium or to leukemia cells treated with or without 50 ng/ml rhIL-17A were determined by cell counting and FCS analysis. c Real-time PCR analysis of the relative mRNA levels of chemokine genes in SupB15 (left) and BV173 cells (right) treated with rhIL-17A (100 ng/ml) or PBS control. d The human IL-5, CXCL5, CCL5 and CXCL16 levels in the culture supernatant of SupB15 cells treated with or without rhIL-17A (100 ng/ml) were measured by ELISA. e The CXCL16 concentrations in the serum of BCR-ABLtTA mice and WT mice (n = 5 mice per group) were quantified with a Mouse Chemokine Assay Q1 (Raybiotech). f The CXCL16 concentrations in the serum of HDs (n = 7 samples)

Article Snippet: Three days after transplantation, 70mg/kg imatinib (Sigma–Aldrich, SML1027, p.o., once a day), 5mg/kg anti-IL-17A neutralizing antibody (Bio X Cell, BP0173, i.v., twice a week), or 0.5mg/kg anti-mouse CXCL16 neutralizing antibody (R&D,MAB503, i.v., twice aweek)were administered for 3 consecutive weeks.

Techniques: Migration, Staining, Cell Counting, Real-time Polymerase Chain Reaction, Control, Enzyme-linked Immunosorbent Assay

Journal: Advanced Science

Article Title: Palmitoyltransferase ZDHHC3 Aggravates Nonalcoholic Steatohepatitis by Targeting S ‐Palmitoylated IRHOM2

doi: 10.1002/advs.202302130

Figure Lengend Snippet: Hepatocyte‐specific loss of Zdhhc3 protects against HFHC‐induced NASH pathogenesis. a‐g) Records for the body weight (a), liver weight and the ratio of liver weight/body weight (%) (LW/BW) (b), fasting blood glucose levels (c), fasting insulin levels (d), HOMA‐IR index (e), glucose tolerance test (GTT) (f) and liver TG, NEFA and TC contents (g) of the HFHC‐fed Zdhhc3 ‐Flox mice and Zdhhc3 ‐HepKO mice; NCD diet‐fed mice were treated as corresponding control ( n = 10 mice per group; P < 0.05 versus Zdhhc3 ‐Flox HFHC group). h) Pearson analysis indicating the correlations between fasting insulin, ratio of liver weight/body weight (%), and liver TG contents in indicated groups ( n = 10 per parameter; P < 0.001 for all of these correlations). i‐k) Representative pictures of H&E staining, oil red O staining, histological NAS score (i) changes, sirius red staining, masson staining (j), and F4/80 and CD11b positive cells expression (k) in indicated groups (magnification, 100× for H&E staining, oil red O staining, masson staining and sirius red staining; magnification, 200× for F4/80 and CD11b staining; n = 10 images per group; P < 0.05 versus Zdhhc3 ‐Flox HFHC group). l, Records for inflammation‐related cytokines profiles including IL‐6, TNF‐α, IL‐1β, IL‐18 and CCL2 in serum from HFHC‐fed HepKO or Flox mice ( n = 10 mice per group; P < 0.05 versus Zdhhc3 ‐Flox HFHC group). m, Records for liver function‐related indicators including ALT, AST, AKP, and GGT in serum from HFHC‐fed HepKO or Flox mice ( n = 10 mice per group; P < 0.05 versus Zdhhc3 ‐Flox HFHC group). Data are expressed as mean ± SEM. The relevant experiments presented in this part were performed independently at least three times. Significance is determined by two‐way analysis of variance (ANOVA) followed by multiple comparisons test analysis (a‐g) or 2‐sided Student's t ‐test (i‐m). The P ‐value < 0.05 was considered as significant difference.

Article Snippet: The TNF‐α (Cat: ab100747), IL‐1β (Cat: ab197742), IL‐6 (Cat: ab222503), CCL2 (Cat: ab208979), IL‐18 (Cat: ab216165) and insulin (Cat: ab285341) ELISA kits were purchased from Abcam.

Techniques: Control, Staining, Expressing